重组人Epigen北京厂家

特别提示：包括重组人Epigen在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：重组人Epigen
英文名称：Recombinant Human Epigen
产品货号：GS4446
产品规格：5μg

分子量：Approximately 7.9kDamonomeric protein,containing 72 amino acid residues,which comprises the EGF homologous portion of the Epigen precursor.
储存条件：冷冻（-20℃）

概述

Epigen is a member of the epidermal growth factor family and is expressed by several tissues,including the testis,liver,heart and in certain tumor cells.It is strongly mitogenic for fibroblasts and epithelial cells,despite its relatively weak affinity for its main receptor,ErbB1.The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion.The gene of human Epigen encodes a 154 amino acid(a.a.)residue type I transmembrane precursor glycoprotein which contains a 22 a.a.signal peptide,an 88 a.a.extracellular domain,a 21 a.a.transmembrane domain and a 23 a.a.cytoplasmic domain.By alternative splicing of the gene,Epigen has several isoforms.The mature,shed form of human Epigen(54 – 104a.a.)shares 92%,94%and 94%a.a.sequence identity with mouse,rat and equine Epigen,respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观	Sterile Filtered White lyophilized(freeze-dried)powder.
纯度	＞98%by SDS-PAGE and HPLC analyses.
来源	Escherichia coli.
生物活性	Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300ng/ml,corresponding to a specific activity of>3.3×103 IU/mg.
成分	Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素	Less than 1 EU/μg of rHuEpigen as determined by LAL method.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列	AVTVTPPITA QQADNIEGPI ALKFSHLCLE DHNSYCINGA CAFHHELEKA ICRCFTGYTG ERCEHLTLTS YA

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