5-Nucleotidase, 5-NT ELISA Kit

Mouse 5- Nucleotidase, 5- NT ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NT5E,CALJA; E5NT; NT5; NTE; eNT; 5'-NT; Purine 5-Prime-Nucleotidase; ecto-5'-nucleotidase; 5'-nucleotidase ecto, 5'-nucleotidase,

Search name

Mouse NT5E ELISA KIT ,Mouse CALJA ELISA KIT ,Mouse E5NT ELISA KIT ,Mouse NT5 ELISA KIT ,Mouse NTE ELISA KIT ,Mouse eNT ELISA KIT ,Mouse 5'-NT ELISA KIT ,Mouse Purine 5-Prime-Nucleotidase ELISA KIT ,Mouse ecto-5'-nucleotidase ELISA KIT ,Mouse 5'-nucleotidase ecto ELISA KIT ,Mouse  5'-nucleotidase ELISA KIT  

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse 5'-nucleotidase,5'-NT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-NT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 5'-NT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 5'-NT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 5'-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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