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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
40S ribosomal protein S17, Rps17
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse 40S ribosomal protein S17, Rps17 ELISA KIT
Product Name:Mouse 40S ribosomal protein S17, Rps17 ELISA KIT
Packing:96T
Catalog No.:ELI-52707m
Gene Name:Mouse Rps17
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Rps17
Alternative Name:Mouse 40S ribosomal protein S17, Rps17
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 40S ribosomal protein S17, Rps17 ELISA KIT allows for the in vitro quantitative determination of Mouse Rps17 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 40S ribosomal protein S17, Rps17 ELISA KIT has been pre-coated with an Mouse 40S ribosomal protein S17, Rps17 antibody specific to Mouse Rps17 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rps17 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rps17 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rps17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验to a basal/housekeeping transcript, e.g., ribosomal protein S17 (RpS17). The TA’s will provide each team with RpS17 primers. For your reference, these are the primer sequences: Forward RpS17: 5' - cga acc aag acg gtg aag aag - 3' Reverse RpS17
the binding sites for the 40S ribosomal subunit and for other translation initiation signals. Alternatively, the formation of a double-stranded DNA/RNA complex can render the RNA susceptible to RNase H digestion (2 ). The antigene approach is based
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
acyltransferase, alpha) (AGPAT1), mRNA 742 NM_021134 Homo sapiens mitochondrial ribosomal protein L23 (MRPL23), mRNA 771 NM_021974 Homo sapiens polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mRNA 1169 NM_006808 Homo sapiens protein
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