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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Queuine tRNA-ribosyltransferase, Qtrt1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Queuine tRNA- ribosyltransferase, QTRT1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse QTRT1 ELISA KIT ,Mouse catalytic subunit ELISA KIT ,Mouse TGT ELISA KIT ,Mouse 43-KD subunit ELISA KIT ,Mouse FP3235 ELISA KIT ,Mouse TGUT ELISA KIT ,Mouse guanine insertion enzyme ELISA KIT ,Mouse queuine tRNA-ribosyltransferase 1 ELISA KIT ,Mouse tRNA-guanine transglycosylase ELISA KIT ,Mouse queuine tRNA-ribosyltransferase catalytic subunit 1 ELISA KIT
Intended use
Mouse QTRT1 ELISA kit allows for the in vitro quantitative determination of Mouse QTRT1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Mouse QTRT1 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for QTRT1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain QTRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of QTRT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse QTRT1 ELISA kit manual request by email:
Order QTRT1 ELISA kit by email:
:
:
: 3128136151
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文献和实验Isolate It All:siRNA • miRNA • Total RNA • Native Protein
? Recover High Quality Total RNA To assess the quality of total RNA isolated with the mir Vana PARIS Kit, 1 x 106 HeLa cells were homogenized in the Cell Disruption Buffer provided with the kit. Because the homogenization is performed quickly
Linear amplification of limiting amounts of RNA for gene expression studies
expression analysis. This approach was successfully applied to gene expression studies of the midbrain-hindbrain organizer region of the mouse but can be applied to many other systems to isolate cell populations. Using the mouse as a model organism
Protocol cDNA-Microarray Hybridization
total Blocking solution (20x stock) 40 µl polydA (1ug/ul) 8 µl tRNA (10ug/ul) 200 µl human/mouse Cot1 DNA (1ug/ul) 240 µl total ethanol precipitate the combined reagents Resuspend in 20 µl filtered ddH2O
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