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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DNA topoisomerase 2-binding protein 1, Topbp1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA topoisomerase 2- binding protein 1, Topbp1 ELISA KIT
Product Name:Mouse DNA topoisomerase 2- binding protein 1, Topbp1 ELISA KIT
Packing:96T
Catalog No.:ELI-51301m
Gene Name:Mouse Topbp1
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Topbp1
Alternative Name:Mouse DNA topoisomerase 2-binding protein 1, Topbp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA topoisomerase 2- binding protein 1, Topbp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Topbp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA topoisomerase 2- binding protein 1, Topbp1 ELISA KIT has been pre-coated with an Mouse DNA topoisomerase 2-binding protein 1, Topbp1 antibody specific to Mouse Topbp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Topbp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Topbp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Topbp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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