Tubulin--tyrosine ligase, Ttl

Mouse Tubulin- - tyrosine ligase, TTL ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Ttl, 2410003M22Rik; tubulin--tyrosine ligase; tubulin-tyrosine ligase; tubulin tyrosine ligase

Search name

Human Ttl ELISA KIT ,Human  2410003M22Rik ELISA KIT ,Human tubulin--tyrosine ligase ELISA KIT ,Human tubulin-tyrosine ligase ELISA KIT ,Human tubulin tyrosine ligase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of  Mouse Tubulin- - tyrosine ligase, TTL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Tubulin- - tyrosine ligase, TTL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Tubulin- - tyrosine ligase, TTL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tubulin- - tyrosine ligase, TTL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Tubulin- - tyrosine ligase, TTL in the samples is then determined by comparing the O.D. of the samples to the standard curve.