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Centrosomal protein of 41 kDa,

Tsga14 ELISA KIT/ Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA试剂盒
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  • ¥2980
  • LSM Bio
  • 78-5000pg/ml
  • 39pg/ml
  • 进口
  • ELI-49966m
  • 2025年07月07日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      50

    • 供应商

      LSM BIO

    • 检测范围

      78-5000pg/ml

    • 检测方法

      夹心法ELISA

    • 应用

      检测小鼠血清,血浆,组织匀浆内的目标蛋白含量

    • 适应物种

      小鼠

    • 标记物

      Centrosomal protein of 41 kDa, Tsga14

    • 样本

      小鼠血清,血浆,组织匀浆

    • 灵敏度

      39pg/ml

    • 规格

      96Tests

    Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA KIT

    Product Name:Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA KIT
    Packing:96T

    Catalog No.:ELI-49966m

    Gene Name:Mouse Tsga14

    Detect Range:78.125-5000pg/ml

    Sensitivity:46.875pg/ml

    Target Protein Name:Mouse Tsga14

    Alternative Name:Mouse Centrosomal protein of 41 kDa, Tsga14
    Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA type:Sandwich ELISA Kit
    Product Description:Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA KIT allows for the in vitro quantitative determination of Mouse Tsga14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

    ELISA Test Principle:
    The microtiter plate provided in Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA KIT has been pre-coated with an Mouse Centrosomal protein of 41 kDa, Tsga14 antibody specific to Mouse Tsga14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tsga14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tsga14 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tsga14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

     


     


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    图标文献和实验
    该产品被引用文献
    Article: Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed swiss albino mice independent of renal lipid signaling Author:Asmaa M.RezkabIslam A.A.E.-H.IbrahimaMona F.MahmoudaAmr A.A.Mahmouda1 Received 6 September 2020, Revised 12 October 2020, Accepted 28 October 2020, Available online 4 November 2020. Chemico-Biological Interactions Available online 4 November 2020, 109307 doi: doi.org/10.1016/j.cbi.2020.109307
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    • [转贴]包涵体纯化复性

      剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择: 尿素和盐酸胍属中强度变性剂,易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用,所需浓度尿素8-10M,盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱,溶解度为70-90%,尿素在作用时间较长或温度较高时会裂解形成氰酸盐,对重组蛋白质的氨

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    文献支持
    Centrosomal protein of 41 kDa, Tsga14 ELISA KIT/ Mouse Centrosomal protein of 41 kDa, Tsga14 ELISA试剂盒
    ¥2980