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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Heat shock protein HSP 90-beta, Hsp90ab1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Heat shock protein HSP 90- beta, Hsp90ab1 ELISA KIT
Product Name:Mouse Heat shock protein HSP 90- beta, Hsp90ab1 ELISA KIT
Packing:96T
Catalog No.:ELI-31341m
Gene Name:Mouse Hsp90ab1
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Hsp90ab1
Alternative Name:Mouse Heat shock protein HSP 90-beta, Hsp90ab1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Heat shock protein HSP 90- beta, Hsp90ab1 ELISA KIT allows for the in vitro quantitative determination of Mouse Hsp90ab1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Heat shock protein HSP 90- beta, Hsp90ab1 ELISA KIT has been pre-coated with an Mouse Heat shock protein HSP 90-beta, Hsp90ab1 antibody specific to Mouse Hsp90ab1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hsp90ab1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hsp90ab1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hsp90ab1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Hsp90 and Client Protein Maturation
Heat-shock protein 90 (Hsp90) is a molecular chaperone that assists in the maturation of a limited set of substrate proteins that are collectively referred to as clients. The majority of identified Hsp90 clients are involved in signal
Detecting HSP90 Phosphorylation
Heat-shock protein 90 (HSP90 ) is an essential molecular chaperone in eukaryotes. It is important for chaperoning proteins that are important determinants of multistep carcinogenesis. HSP90 ’s ATPase activity is associated with its chaperone
Analysis of Heat-Shock Protein Localisation Using Flow Cytometry
The use of flow cytometry in heat-shock protein (HSP) research is increasing rapidly due to the high sensitivity and versatility of the technique. The method allows the simultaneous analysis of multiple proteins within numerous cell types
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