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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DNA-3-methyladenine glycosylase, Mpg
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA- 3- methyladenine glycosylase, Mpg ELISA KIT
Product Name:Mouse DNA- 3- methyladenine glycosylase, Mpg ELISA KIT
Packing:96T
Catalog No.:ELI-49804m
Gene Name:Mouse Mpg
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Mpg
Alternative Name:Mouse DNA-3-methyladenine glycosylase, Mpg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA- 3- methyladenine glycosylase, Mpg ELISA KIT allows for the in vitro quantitative determination of Mouse Mpg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA- 3- methyladenine glycosylase, Mpg ELISA KIT has been pre-coated with an Mouse DNA-3-methyladenine glycosylase, Mpg antibody specific to Mouse Mpg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mpg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mpg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mpg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Reproducible purification of DNA and RNA in 96-well format. Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. A Purified DNA samples were analyzed on a 0.8% agarose gel. B
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
在RT-PCR中避免DNA污染(Avoiding DNA Contamination in R
. The PCR products in the "no-RT" samples are the result of amplification from trace amounts of genomic contamination. Figure 1. DNA Contamination in RNA Isolated by Five Different Methods. Mouse liver total RNA
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