Chromatin complexes subunit BA
P18, Bap18 ELISA KIT/ Mouse Chromatin complexes subunit BAP18, Bap18 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-49630m
- 2026年02月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Chromatin complexes subunit BAP18, Bap18
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Chromatin complexes subunit BAP18, Bap18 ELISA KIT
Product Name:Mouse Chromatin complexes subunit BAP18, Bap18 ELISA KIT
Packing:96T
Catalog No.:ELI-49630m
Gene Name:Mouse Bap18
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Bap18
Alternative Name:Mouse Chromatin complexes subunit BAP18, Bap18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Chromatin complexes subunit BAP18, Bap18 ELISA KIT allows for the in vitro quantitative determination of Mouse Bap18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Chromatin complexes subunit BAP18, Bap18 ELISA KIT has been pre-coated with an Mouse Chromatin complexes subunit BAP18, Bap18 antibody specific to Mouse Bap18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Bap18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Bap18 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Bap18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Chromatin Immunoprecipitation (ChIP)
for fragmenting chromatin for N-ChIP, whereas chromatin for X-ChIP is usually sonicated, sometimes in conjunction with MNase digestion. 5. One of the major limitations of ChIP is the need for antibodies that recognize the target protein
Native Chromatin Preparation and Illumina/Solexa Library Construction
is empty. Collect the entire effluent in the same tube (from Step 18). 20. Count cells and check CD4+ T cell purity. Recovery is typically ~20%. Purified CD4+ T cells with ~ 95%-98% purity are used for native chromatin preparation
PCR purification kit) purify DNA through Qiaquick column elute DNA into 50 µl H2 O use 0.5 - 1 µl per 25 µl PCR reaction: 1 x 95 °C, 2 min 21 x 95 °C, 30 sec; 60 °C, 30 sec; 72 °C, 1 min 1 x 72
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