Mouse Cysteine protease ATG4B,

Mouse Cysteine protease ATG4B, Atg4b ELISA KIT

Product Name:Mouse Cysteine protease ATG4B, Atg4b ELISA KIT
Packing:96T

Catalog No.:ELI-48997m

Gene Name:Mouse Atg4b

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Atg4b

Alternative Name:Mouse Cysteine protease ATG4B, Atg4b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cysteine protease ATG4B, Atg4b ELISA KIT allows for the in vitro quantitative determination of Mouse Atg4b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Cysteine protease ATG4B, Atg4b ELISA KIT has been pre-coated with an Mouse Cysteine protease ATG4B, Atg4b antibody specific to Mouse Atg4b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Atg4b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Atg4b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Atg4b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!