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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Histone deacetylase 9, Hdac9
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Histone deacetylase 9, Hdac9 ELISA KIT
Product Name:Mouse Histone deacetylase 9, Hdac9 ELISA KIT
Packing:96T
Catalog No.:ELI-48598m
Gene Name:Mouse Hdac9
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Hdac9
Alternative Name:Mouse Histone deacetylase 9, Hdac9
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone deacetylase 9, Hdac9 ELISA KIT allows for the in vitro quantitative determination of Mouse Hdac9 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histone deacetylase 9, Hdac9 ELISA KIT has been pre-coated with an Mouse Histone deacetylase 9, Hdac9 antibody specific to Mouse Hdac9 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hdac9 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hdac9 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hdac9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【求助】一句英文怎么翻译?涉及TGFβ-b诱导乙酰化的Foxp3 。谢谢!
,也占用了IL-2的启动子区域,从而抑制IL-2的转录。 背景介绍一下:其实在正常情况下,转录因子Foxp3是启动IL-2的转录,但是使用trichostatin(是一种常用的HDAC(histone deacetylase)抑制剂。Trichostatin A可以通透细胞,选择性抑制HDACs,但不抑制NAD依赖的SIRT系列的deacetylases。)促进Foxp3的表达的同时,也促进了Foxp3的乙酰化,乙酰化的Foxp3增强了其结合在IL-2启动子的能力,但是抑制IL-2的转录
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