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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Dermatan-sulfate epimerase-like protein, Dsel
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Dermatan- sulfate epimerase- like protein, Dsel ELISA KIT
Product Name:Mouse Dermatan- sulfate epimerase- like protein, Dsel ELISA KIT
Packing:96T
Catalog No.:ELI-48164m
Gene Name:Mouse Dsel
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dsel
Alternative Name:Mouse Dermatan-sulfate epimerase-like protein, Dsel
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dermatan- sulfate epimerase- like protein, Dsel ELISA KIT allows for the in vitro quantitative determination of Mouse Dsel concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dermatan- sulfate epimerase- like protein, Dsel ELISA KIT has been pre-coated with an Mouse Dermatan-sulfate epimerase-like protein, Dsel antibody specific to Mouse Dsel .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dsel and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dsel , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dsel in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
mm (100) - ESCO (VWR 9611301) Dextran sulfate - Sigma® 31403 Formamide - Sigma® F 5786 Gelatin 250g - Merck® (VWR 24360233) Glycerol - Sigma® G5516 Mouse X-chromosome paint - Cambio 1187-XMB-02 Paraformaldehyde - RE/PURO 387507 PBS 10X 500ml
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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