Growth factor receptor-bound p

Mouse Growth factor receptor- bound protein 14, Grb14 ELISA KIT

Product Name:Mouse Growth factor receptor- bound protein 14, Grb14 ELISA KIT
Packing:96T

Catalog No.:ELI-47994m

Gene Name:Mouse Grb14

Detect Range:31.2-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Grb14

Alternative Name:Mouse Growth factor receptor-bound protein 14, Grb14
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Growth factor receptor- bound protein 14, Grb14 ELISA KIT allows for the in vitro quantitative determination of Mouse Grb14 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Growth factor receptor- bound protein 14, Grb14 ELISA KIT has been pre-coated with an Mouse Growth factor receptor-bound protein 14, Grb14 antibody specific to Mouse Grb14 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Grb14 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Grb14 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Grb14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!