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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Potassium voltage-gated channel subfamily B member 2, Kcn
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Potassium voltage-gated channel subfamily B member 2, KCNB2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Potassium voltage-gated channel subfamily B member 2,Voltage-gated potassium channel subunit Kv2.2, KCNB2,KV2.2,delayed rectifier potassium channel protein,potassium channel Kv2.2,potassium voltage-gated channel subfamily B member 2,voltage-gated potassium channel subunit Kv2.2,potassium voltage-gated channel, Shab-related subfamily member 2
Search name
Mouse Potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Mouse Voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Mouse KCNB2 ELISA KIT ,Mouse KV2.2 ELISA KIT ,Mouse delayed rectifier potassium channel protein ELISA KIT ,Mouse potassium channel Kv2.2 ELISA KIT ,Mouse potassium voltage-gated channel subfamily B member 2 ELISA KIT ,Mouse voltage-gated potassium channel subunit Kv2.2 ELISA KIT ,Mouse potassium voltage-gated channel ELISA KIT ,Mouse Shab-related subfamily member 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse potassium voltage-gated channel subfamily b member 2,KCNB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to KCNB2, During the reaction, KCNB2 in the sample or standard competes with a fixed amount of biotin-labeled KCNB2 for sites on a pre-coated Monoclonal antibody specific to KCNB2. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of KCNB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Potassium Channel Binding
Figure 1.17.1 Displacement of [3 H]P 1075 whole‐cell binding to KATP channels in rat A10 cells using P 1075 and two potassium channel–opener compounds. The data was fitted using GraphPad Prism program. Figure kindly contributed by R. Davis Taber
The Loose Patch Voltage Clamp Technique
until it was revived by St�hmer and Almers (1982). Almers, St�hmer, and their collaborators used the loose patch-clamp to study a wide range of muscle sodium and potassium channel properties, including mobility, inactivation, and spatial distribution (St�hmer
Characterization of Calcium Channel Binding
.T., Naylor, M.J., Maybaum, T.A., Pearce, W.G., Koop, B., Fishman, G.A., Mets, M., Musarella, M.A., and Boycott, K.M. 1998. Loss‐of‐function mutations in a calcium‐channel alpha1‐subunit gene in Xp11.23 cause incomplete X‐linked congenital stationary
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