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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Dystrobrevin beta, Dtnb
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Dystrobrevin beta, Dtnb ELISA KIT
Product Name:Mouse Dystrobrevin beta, Dtnb ELISA KIT
Packing:96T
Catalog No.:ELI-47723m
Gene Name:Mouse Dtnb
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Dtnb
Alternative Name:Mouse Dystrobrevin beta, Dtnb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Dystrobrevin beta, Dtnb ELISA KIT allows for the in vitro quantitative determination of Mouse Dtnb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Dystrobrevin beta, Dtnb ELISA KIT has been pre-coated with an Mouse Dystrobrevin beta, Dtnb antibody specific to Mouse Dtnb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dtnb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dtnb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dtnb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验FA Extraction of [insoluble] Amyloid beta from Mouse Brain
each sample individually for one minute on ice. Immerse the tip of the probe in the sample, then move tube up and down while sonicating. 3. Spin 400 μl at 135,000 x g for 1 hr at 4°C (50,000 rpm in a TLA 100.3 rotor). 4. Dilute 210 μl
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Antigen Retrieval : for immunohistochemistry (Mike A. Dyer) Dissociated Cell Staining : immunohistochemistry (Mike A. Dyer) 相关链接: 仪器库 >> 显微系统 >> 倒置显微镜 仪器库 >> 影像系统 >>专业影像系统 试剂库 >> 免疫检测 >>ELISA检测试剂盒 抗体库 >> 抗体 抗体
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