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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Collagen alpha-2(V) chain, Col5a2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Collagen alpha- 2(V) chain, Col5a2 ELISA KIT
Product Name:Mouse Collagen alpha- 2(V) chain, Col5a2 ELISA KIT
Packing:96T
Catalog No.:ELI-46749m
Gene Name:Mouse Col5a2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Col5a2
Alternative Name:Mouse Collagen alpha-2(V) chain, Col5a2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Collagen alpha- 2(V) chain, Col5a2 ELISA KIT allows for the in vitro quantitative determination of Mouse Col5a2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Collagen alpha- 2(V) chain, Col5a2 ELISA KIT has been pre-coated with an Mouse Collagen alpha-2(V) chain, Col5a2 antibody specific to Mouse Col5a2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Col5a2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Col5a2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Col5a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The enzyme-linked immunosorbent assay (ELISA) is a widely used technique for detecting antibodies (Abs) and is employed in clinical laboratories to identify Abs against various self-antigens–autoAb development and quantitation. This method
V294.PART II,Chapter 5 细胞粘附实验(英文版)
variations to thebasic protocols and areas where special attentions are required for successfulapplication of the methods. 2. Materials 2.1. Static Adhesion Assays 1. 96-Well enzyme-linked immunosorbent assay (ELISA) plates (Corning
has also been found in rats in which EAE was induced by a peptide of myelin basic protein (5 ). Similarly, a restriction in the TCR usage was found also in CII-induced arthritis in mice (6 ,7 ), and in the TCR alpha chain usage in non-obese diabetic (NOD) mice (8 ).
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