General transcription factor 3
C polypeptide 1, Gtf3c1 ELIS/ Mouse General transcription factor 3C polypeptide 1, Gtf3c1 ELIS- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-46556m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
General transcription factor 3C polypeptide 1, Gtf3c1 ELIS
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse General transcription factor 3C polypeptide 1, Gtf3c1 ELIS
Product Name:Mouse General transcription factor 3C polypeptide 1, Gtf3c1 ELIS
Packing:96T
Catalog No.:ELI-46556m
Gene Name:Mouse Gtf3c1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse General transcription factor 3C polypeptide 1
Alternative Name:Mouse Gtf3c1 ELISA KIT, Mouse General transcription factor 3C polypeptide 1 ELISA KIT
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse General transcription factor 3C polypeptide 1, Gtf3c1 ELIS allows for the in vitro quantitative determination ofMouse General transcription factor 3C polypeptide 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse General transcription factor 3C polypeptide 1, Gtf3c1 ELIS has been pre-coated with an Mouse Gtf3c1 antibody specific to Mouse General transcription factor 3C polypeptide 1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse General transcription factor 3C polypeptide 1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse General transcription factor 3C polypeptide 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse General transcription factor 3C polypeptide 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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X transcription buffer (Stratagene) 2 µl labelling mix 1 µl RNAGUARD (Pharmacia) 1 µl RNA polymerase (SP6, T3 or T7) Add water to 20 µl Incubate for 2 hours at 37 °C. Run on 1% agarose gel (1.5µl
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