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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Fibrocystin-L, Pkhd1l1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Fibrocystin- L, Pkhd1l1 ELISA KIT
Product Name:Mouse Fibrocystin- L, Pkhd1l1 ELISA KIT
Packing:96T
Catalog No.:ELI-45328m
Gene Name:Mouse Pkhd1l1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pkhd1l1
Alternative Name:Mouse Fibrocystin-L, Pkhd1l1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Fibrocystin- L, Pkhd1l1 ELISA KIT allows for the in vitro quantitative determination of Mouse Pkhd1l1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Fibrocystin- L, Pkhd1l1 ELISA KIT has been pre-coated with an Mouse Fibrocystin-L, Pkhd1l1 antibody specific to Mouse Pkhd1l1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pkhd1l1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pkhd1l1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pkhd1l1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验本文主要介绍 ELISA 试剂盒、实验样本、相关问题,帮助大家更好地开展实验 试剂盒 Q1:科研试剂盒与临床试剂盒的区别 ? A1:临床试剂是经过权威机构认可的,如 FDA、CFDA 等;而科研试剂一般是厂家自主研制,不需要通过权威机构验证。 经营临床诊断类产品的机构需要取得《医疗器械经营企业许可证》;经营科研试剂的机构不需要此证件。 临床试剂盒一般仅用于检测人血清/血浆等较为单一的分泌性样本类型;科研试剂盒的样本检测种类、检测范围则比临床试剂盒宽广得多。但一般来说,临床试剂盒的质量
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit(cat NO:EKS-420A)?可否说一下应用的经验?注意事项等?还有,该试剂盒的说明书有几处地方不是很懂,请大家帮忙看一下: 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
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