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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ribonuclease P protein subunit p30, Rpp30
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ribonuclease P protein subunit p30, Rpp30 ELISA KIT
Product Name:Mouse Ribonuclease P protein subunit p30, Rpp30 ELISA KIT
Packing:96T
Catalog No.:ELI-45292m
Gene Name:Mouse Rpp30
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Rpp30
Alternative Name:Mouse Ribonuclease P protein subunit p30, Rpp30
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ribonuclease P protein subunit p30, Rpp30 ELISA KIT allows for the in vitro quantitative determination of Mouse Rpp30 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ribonuclease P protein subunit p30, Rpp30 ELISA KIT has been pre-coated with an Mouse Ribonuclease P protein subunit p30, Rpp30 antibody specific to Mouse Rpp30 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rpp30 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rpp30 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rpp30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验occurs with 1∶1 stoichiometry. A subset of the pancreatic ribonuclease superfamily, including the amphibian ribonucleases such as frog-liver ribonuclease, sialic acid-binding lectin, and P-30 protein, are not inhibited by RI. The potent inhibitory
Ribonuclease P (RNase P) from Escherichia coli is a transfer RNA (tRNA)-processing enzyme and consists of a catalytic RNA subunit (M1 RNA) and a protein component (C5 protein). M1GS, a gene-targeting ribozyme derived from M1 RNA, can cleave
subunits homologous to eukaryotic nuclear RNase P proteins. RNA. 2002, 8 (3): 296-306. PMID 12003490. ^ (英文)Fukuhara H, Kifusa M, Watanabe M, Terada A, Honda T, Numata T, Kakuta Y, Kimura M. A fifth protein subunit Ph1496p elevates the optimum
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