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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
POC1 centriolar protein homolog B, Poc1b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse POC1 centriolar protein homolog B, Poc1b ELISA KIT
Product Name:Mouse POC1 centriolar protein homolog B, Poc1b ELISA KIT
Packing:96T
Catalog No.:ELI-45242m
Gene Name:Mouse Poc1b
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Poc1b
Alternative Name:Mouse POC1 centriolar protein homolog B, Poc1b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse POC1 centriolar protein homolog B, Poc1b ELISA KIT allows for the in vitro quantitative determination of Mouse Poc1b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse POC1 centriolar protein homolog B, Poc1b ELISA KIT has been pre-coated with an Mouse POC1 centriolar protein homolog B, Poc1b antibody specific to Mouse Poc1b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Poc1b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Poc1b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Poc1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Kim, J., Cantor, A.B., Orkin, S.H., and Wang, J. 2009. Use of in vivo biotinylation to study protein‐protein and protein‐DNA interactions in mouse embryonic stem cells. Nat. Protoc. 4:506‐517
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Synthesis of Peptide‐Oligonucleotide Conjugates Using a Heterobifunctional Crosslinker
Williams, B.A.R., Diehnelt, C.W., Belcher, P., Greving, M., Woodbury, N.W., Johnston, S.A., and Chaput, J.C. 2009. Creating protein affinity reagents by combining peptide ligands on synthetic DNA scaffolds. J. Am. Chem. Soc. 131:17233
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