- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-45000m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Peptidase inhibitor 15, Pi15
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Peptidase inhibitor 15, Pi15 ELISA KIT
Product Name:Mouse Peptidase inhibitor 15, Pi15 ELISA KIT
Packing:96T
Catalog No.:ELI-45000m
Gene Name:Mouse Pi15
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Pi15
Alternative Name:Mouse Peptidase inhibitor 15, Pi15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Peptidase inhibitor 15, Pi15 ELISA KIT allows for the in vitro quantitative determination of Mouse Pi15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Peptidase inhibitor 15, Pi15 ELISA KIT has been pre-coated with an Mouse Peptidase inhibitor 15, Pi15 antibody specific to Mouse Pi15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pi15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pi15 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pi15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Expanding Mouse Ventricular Cardiomyocytes Through GSK‐3 Inhibition
embryos. (B ) Mouse embryo at ∼E12.5. Dashed lines indicate where incisions should be made to yield the heart. (C ) Fetal mouse heart. Dashed lines indicate excision of atrial tissue. (D ) Pooling of ventricular tissue in 15‐ml tubes. Two to three tubes
or 4°C; Wash 3 times in 1X PBS for 5 minutes each; Permeabilize with freshly made 1X PBS/0.5% Triton X-100 (containing the RNAse-inhibitor, 2mM Vanadyl Ribonucleoside Complex, in case of a subsequent RNA-FISH) on ice for 3.5-5 minutes
. In the first, ROCK inhibitor (ROCKi) was used, which allows accurate counting and plating of single hESC. In the second, small colonies were plated without ROCKi but with adaptations for accurate counting and plating. The MTT assay was also adapted for use with mouse
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