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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Signal peptide peptidase-like 3, Sppl3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Signal peptide peptidase- like 3, Sppl3 ELISA KIT
Product Name:Mouse Signal peptide peptidase- like 3, Sppl3 ELISA KIT
Packing:96T
Catalog No.:ELI-44837m
Gene Name:Mouse Sppl3
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Sppl3
Alternative Name:Mouse Signal peptide peptidase-like 3, Sppl3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Signal peptide peptidase- like 3, Sppl3 ELISA KIT allows for the in vitro quantitative determination of Mouse Sppl3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Signal peptide peptidase- like 3, Sppl3 ELISA KIT has been pre-coated with an Mouse Signal peptide peptidase-like 3, Sppl3 antibody specific to Mouse Sppl3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sppl3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sppl3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sppl3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Synthesis and Probing of Membrane-bound Peptide Arrays
SPOT synthesis kit: A kit that includes all necessary items for manual SPOT synthesis is available from Sigma-Genosys. Software for the generation of peptide lists and pipetting protocols is included in the Sigma-Genosys
Specificity Profiling of Protein‐Binding Domains Using One‐Bead‐One‐Compound Peptide Libraries
‐31276. Ekici, O.D., Karla, A., Paetzel, M., Lively, M.O., Pei, D.H., and Dalbey, R.E. 2007. Altered‐3 substrate specificity of Escherichia coli signal peptidase 1 mutants
Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
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