- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-44627m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine a
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Peptide- N(4)- (N- acetyl- beta- glucosaminyl) asparagine amidase, NGLY1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NGLY1,CDG1V; PNG1; PNGase; hPNGase; peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase; peptide:N-glycanase; N-glycanase 1
Search name
Mouse NGLY1 ELISA KIT ,Mouse CDG1V ELISA KIT ,Mouse PNG1 ELISA KIT ,Mouse PNGase ELISA KIT ,Mouse hPNGase ELISA KIT ,Mouse peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase ELISA KIT ,Mouse peptide:N-glycanase ELISA KIT ,Mouse N-glycanase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Peptide- N(4)- (N- acetyl- beta- glucosaminyl) asparagine amidase, NGLY1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NGLY1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NGLY1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NGLY1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NGLY1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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: 3128136151
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Peptide Inhibition ELISA Protocol
Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe. Add an equal volume of PBT and MAb as the control. Incubate 1 hour at 37
Peptide Inhibition ELISA Protocol
Peptide Inhibition ELISA Protocol Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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