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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Nuclear pore complex protein Nup93, Nup93
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT
Product Name:Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT
Packing:96T
Catalog No.:ELI-44561m
Gene Name:Mouse Nup93
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Nup93
Alternative Name:Mouse Nuclear pore complex protein Nup93, Nup93
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT allows for the in vitro quantitative determination of Mouse Nup93 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Nuclear pore complex protein Nup93, Nup93 ELISA KIT has been pre-coated with an Mouse Nuclear pore complex protein Nup93, Nup93 antibody specific to Mouse Nup93 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nup93 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nup93 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nup93 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验施一公团队突破性进展再登 Science!以超高分辨率解析核孔复合物的结构细节
质组装成正确的三维分子结构一直是一个巨大的挑战。 2022 年 6 月 10 日,西湖大学施一公团队在 Science 杂志发表研究论文 Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex。 该研究聚焦于非洲爪蟾核孔复合物(NPC)结构,首次扩展了脊椎动物细胞质环(CR)亚单位复合体模型的分子量,获得高分辨率的单粒子冷冻电镜显微结构。除了解析 Y 复合物,同时发现 5 个核孔蛋白 Nup358
Reconstitution of Nuclear Import in Permeabilized Cells
factors. The latter include receptors (karyopherins) that recognize the cargoes and carry them across the nuclear pore complex (NPC), and the small GTPase Ran, which modulates karyopherin–cargo binding. Nuclear import can be studied in vitro using
Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. Nuclear export has been suggested
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