Protein phosphatase 1G, Ppm1g
ELISA KIT/ Mouse Protein phosphatase 1G, Ppm1g ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-43110m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Protein phosphatase 1G, Ppm1g
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein phosphatase 1G, Ppm1g ELISA KIT
Product Name:Mouse Protein phosphatase 1G, Ppm1g ELISA KIT
Packing:96T
Catalog No.:ELI-43110m
Gene Name:Mouse Ppm1g
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ppm1g
Alternative Name:Mouse Protein phosphatase 1G, Ppm1g
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein phosphatase 1G, Ppm1g ELISA KIT allows for the in vitro quantitative determination of Mouse Ppm1g concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein phosphatase 1G, Ppm1g ELISA KIT has been pre-coated with an Mouse Protein phosphatase 1G, Ppm1g antibody specific to Mouse Ppm1g .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ppm1g and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ppm1g , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ppm1g in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Purification of acidic phosphatase from mustard seedlings
and record the weight. (use 25-50 g). 2. Grind in a mortar with 75 ml of ice-cold water. 3. Make the volume up to 150 ml and transfer the slurry into the Polytron homogenizer. Homogenize 1 min at speed 5 (3x20 sec). 4. Filter
Purification of acidic phosphatase from mustard seedlings
6. Centrifuge in the Sorvall superspeed centrifuge (B 7202) in GSA rotor (r = 12.5 cm) at 9500 rpm (1,000 x g) for 30 min. 7. Decant the supernatant (SN1) (contains many organelles and the cytosol) into a graduated cylinder and note the volume. Remove
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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