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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Sorting nexin-5, Snx5
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Sorting nexin- 5, Snx5 ELISA KIT
Product Name:Mouse Sorting nexin- 5, Snx5 ELISA KIT
Packing:96T
Catalog No.:ELI-41492m
Gene Name:Mouse Snx5
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Snx5
Alternative Name:Mouse Sorting nexin-5, Snx5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Sorting nexin- 5, Snx5 ELISA KIT allows for the in vitro quantitative determination of Mouse Snx5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Sorting nexin- 5, Snx5 ELISA KIT has been pre-coated with an Mouse Sorting nexin-5, Snx5 antibody specific to Mouse Snx5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Snx5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Snx5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Snx5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
(yellow when acid is added). 2) Coat plate with antigen stock solution (1ug/ml in PBS, 50ul/well) for at least 24hrs or over the weekend at 4°C or O/N at 37°C. 3) Remove antigen and wash 3x with 1x PBS. 4) Block with 1% BSA (100ul/well) in 1x PBS
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
ELISA (Enzyme-Linked ImmunoadSorbent Assay)You will need the following solutions:PBS (Phosphate-Buffered Saline, Ca/Mg free) :-Solute 1x 10xNaCl 8g 80gKCl 0.2g 2gNa2PO4 (anhydrous) 1.15g 11.5gKH2PO4 (anhydrous) 0.2g 2gN.B.: Na2HPO4.2
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
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