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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
T-cell receptor alpha chain C region, Tcra
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse T- cell receptor alpha chain C region, Tcra ELISA KIT
Product Name:Mouse T- cell receptor alpha chain C region, Tcra ELISA KIT
Packing:96T
Catalog No.:ELI-41392m
Gene Name:Mouse Tcra
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Tcra
Alternative Name:Mouse T-cell receptor alpha chain C region, Tcra
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse T- cell receptor alpha chain C region, Tcra ELISA KIT allows for the in vitro quantitative determination of Mouse Tcra concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse T- cell receptor alpha chain C region, Tcra ELISA KIT has been pre-coated with an Mouse T-cell receptor alpha chain C region, Tcra antibody specific to Mouse Tcra .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tcra and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tcra , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tcra in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验encephalomyelitis (EAE)—a model of multiple sclerosis (MS), collagen II (CII)-induced arthritis (CIA)—a model of rheumatoid arthritis (RA). Pathogenic T cells in EAE utilize a restricted repertoire of genes encoding the T-cell receptor (TCR) (1 ). For example
Molecular Measurement of T Cell Receptor Excision Circles
This chapter provides protocols necessary for quantifying human, mouse, and nonhuman primate signal joint T cell receptor excision circles (sjTRECs) produced during T cell receptor alpha (TCRA) gene rearrangement. These non-replicated
randomly paired light (L) and heavy (H) chain coding regions under transcriptional control of P lac . The H chain coding region is fused in-frame with the phage gene, Δ gIII , coding for a truncated version of the phage surface protein pIII (ΔpIII
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