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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Troponin C, skeletal muscle, Tnnc2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Troponin C, skeletal muscle, Tnnc2 ELISA KIT
Product Name:Mouse Troponin C, skeletal muscle, Tnnc2 ELISA KIT
Packing:96T
Catalog No.:ELI-39994m
Gene Name:Mouse Tnnc2
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Tnnc2
Alternative Name:Mouse Troponin C, skeletal muscle, Tnnc2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Troponin C, skeletal muscle, Tnnc2 ELISA KIT allows for the in vitro quantitative determination of Mouse Tnnc2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Troponin C, skeletal muscle, Tnnc2 ELISA KIT has been pre-coated with an Mouse Troponin C, skeletal muscle, Tnnc2 antibody specific to Mouse Tnnc2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tnnc2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tnnc2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tnnc2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
:
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文献和实验troponin蛋白纯化 Protein purification: troponins
regulatory mechanism for muscle contration. Material Source: skeletal muscle ether powder Equipment: • centrifuge • Cibaron Blue-sephacryl column (5 x 50 cm) • DEAE-sephadex A-50 column (2.5 x 25 cm) • CM-sephadex C-50-120 column
terminals. J. Neurosci. 12:363‐375. Brown, M.C., Jansen, J.K., and Van Essen, D. 1976. Polyneuronal innervation of skeletal muscle in new‐born rats and its elimination
Systematic Evaluation of Skeletal Mechanical Function
Figure 2. Sectioning of mouse femora. (A ) Wide view of IsoMet diamond wafering saw. (B ) Enlarged view of chuck, specimen, and blade interface. (C ) Single femur embedded in polymerized MMA
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