M-phase inducer phosphatase 1,
Cdc25a ELISA KIT/ Mouse M-phase inducer phosphatase 1, Cdc25a ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-38523m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
M-phase inducer phosphatase 1, Cdc25a
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse M- phase inducer phosphatase 1, Cdc25a ELISA KIT
Product Name:Mouse M- phase inducer phosphatase 1, Cdc25a ELISA KIT
Packing:96T
Catalog No.:ELI-38523m
Gene Name:Mouse Cdc25a
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cdc25a
Alternative Name:Mouse M-phase inducer phosphatase 1, Cdc25a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse M- phase inducer phosphatase 1, Cdc25a ELISA KIT allows for the in vitro quantitative determination of Mouse Cdc25a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse M- phase inducer phosphatase 1, Cdc25a ELISA KIT has been pre-coated with an Mouse M-phase inducer phosphatase 1, Cdc25a antibody specific to Mouse Cdc25a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cdc25a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cdc25a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cdc25a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
cell genome and is thereafter referred to as a "provirus". Integration of the genome of most retroviruses requires that the cell go through an M phase [2], and thus only mitotic cells will serve successfully as hosts for integration of most retroviruses
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