M-phase inducer phosphatase 3,
Cdc25c ELISA KIT/ Mouse M-phase inducer phosphatase 3, Cdc25c ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14232m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
M-phase inducer phosphatase 3, Cdc25c
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse M- phase inducer phosphatase 3, CDC25C ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC25C,dual specificity phosphatase CDC25C; CDC25; PPP1R60; M-phase inducer phosphatase 3; mitosis inducer CDC25; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60; cell division cycle 25C
Search name
Mouse CDC25C ELISA KIT ,Mouse dual specificity phosphatase CDC25C ELISA KIT ,Mouse CDC25 ELISA KIT ,Mouse PPP1R60 ELISA KIT ,Mouse M-phase inducer phosphatase 3 ELISA KIT ,Mouse mitosis inducer CDC25 ELISA KIT ,Mouse phosphotyrosine phosphatase ELISA KIT ,Mouse protein phosphatase 1 ELISA KIT ,Mouse regulatory subunit 60 ELISA KIT ,Mouse cell division cycle 25C ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse M- phase inducer phosphatase 3, CDC25C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to M-phase inducer phosphatase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-phase inducer phosphatase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain M-phase inducer phosphatase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of M-phase inducer phosphatase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
Use of Xenopus Oocytes and Early Embryos to Study MAPK Signaling
in an inactive form (pre-MPF) during the G2 arrest owing to the phosphorylation of Cdc2 by the Myt1 protein kinase. The dephosphorylation necessary to activate pre-MPF and induce progression of the oocyte into meiosis is catalyzed by the phosphatase Cdc25C.
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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