Mouse M- phase inducer phosphatase 3, CDC25C ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC25C,dual specificity phosphatase CDC25C; CDC25; PPP1R60; M-phase inducer phosphatase 3; mitosis inducer CDC25; phosphotyrosine phosphatase; protein phosphatase 1, regulatory subunit 60; cell division cycle 25C
Search name
Mouse CDC25C ELISA KIT ,Mouse dual specificity phosphatase CDC25C ELISA KIT ,Mouse CDC25 ELISA KIT ,Mouse PPP1R60 ELISA KIT ,Mouse M-phase inducer phosphatase 3 ELISA KIT ,Mouse mitosis inducer CDC25 ELISA KIT ,Mouse phosphotyrosine phosphatase ELISA KIT ,Mouse protein phosphatase 1 ELISA KIT ,Mouse regulatory subunit 60 ELISA KIT ,Mouse cell division cycle 25C ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse M- phase inducer phosphatase 3, CDC25C concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to M-phase inducer phosphatase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-phase inducer phosphatase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain M-phase inducer phosphatase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of M-phase inducer phosphatase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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