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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein kinase C gamma type, Prkcg
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein kinase C gamma type, Prkcg ELISA KIT
Product Name:Mouse Protein kinase C gamma type, Prkcg ELISA KIT
Packing:96T
Catalog No.:ELI-37807m
Gene Name:Mouse Prkcg
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Prkcg
Alternative Name:Mouse Protein kinase C gamma type, Prkcg
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein kinase C gamma type, Prkcg ELISA KIT allows for the in vitro quantitative determination of Mouse Prkcg concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein kinase C gamma type, Prkcg ELISA KIT has been pre-coated with an Mouse Protein kinase C gamma type, Prkcg antibody specific to Mouse Prkcg .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prkcg and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prkcg , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prkcg in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
We previously isolated Aurora-C (Aurkc/Aie1 ) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment
Angiotensin Protein Kinase Assay
in 12 microliters kinase buffer (40 mM PIPES, pH 7.0, 10 mM MnCl2) at 4°C. 2. The kinase reaction is carried out at room temperature in a total volume of 20 microliters that contains: 15 microCi gamma-32P-ATP 5 microliters
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