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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Protein kinase C alpha type, Prkca含量
- 适应物种:
小鼠
- 标记物:
Protein kinase C alpha type, Prkca
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein kinase C alpha type, Prkca ELISA试剂盒
Product Name: Mouse Protein kinase C alpha type, Prkca ELISA试剂盒
Product Name: Mouse Protein kinase C alpha type, Prkca ELISA试剂盒
Gene Name: Mouse Protein kinase C alpha type, Prkca
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Protein kinase C alpha type, Prkca
Alternative Name: Mouse Protein kinase C alpha type, Prkca
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Protein kinase C alpha type, Prkca ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Protein kinase C alpha type, Prkca concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Protein kinase C alpha type, Prkca ELISA试剂盒 has been pre-coated with an Mouse Protein kinase C alpha type, Prkca antibody specific to Mouse Protein kinase C alpha type, Prkca .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Protein kinase C alpha type, Prkca and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Protein kinase C alpha type, Prkca , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Protein kinase C alpha type, Prkca in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
We previously isolated Aurora-C (Aurkc/Aie1 ) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
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