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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
GC-rich sequence DNA-binding factor 1, Gcfc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT
Product Name:Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT
Packing:96T
Catalog No.:ELI-37633m
Gene Name:Mouse Gcfc1
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Gcfc1
Alternative Name:Mouse GC-rich sequence DNA-binding factor 1, Gcfc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Gcfc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse GC- rich sequence DNA- binding factor 1, Gcfc1 ELISA KIT has been pre-coated with an Mouse GC-rich sequence DNA-binding factor 1, Gcfc1 antibody specific to Mouse Gcfc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gcfc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gcfc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gcfc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Production of Monoclonal Antibody by DNA Immunization with Electroporation
against a house dust mite allergen, designated as Blo t 11, which is a paramyosin homologue found in Blomia tropicalis mites. The Blo t 11 cDNA fused at the N terminus to the sequence of a signal peptide was cloned into the pCI mammalian expression vector. The DNA
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
of the gene sequence. We have not seen any correlation between the position of target sites on the mRNA and siRNA potency.Compare the potential target sites to the appropriate genome database (human, mouse, rat, etc.) and eliminate from consideration
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