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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Gamma-secretase subunit APH-1B, Aph1b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Gamma- secretase subunit APH- 1B, Aph1b ELISA KIT
Product Name:Mouse Gamma- secretase subunit APH- 1B, Aph1b ELISA KIT
Packing:96T
Catalog No.:ELI-34843m
Gene Name:Mouse Aph1b
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Aph1b
Alternative Name:Mouse Gamma-secretase subunit APH-1B, Aph1b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Gamma- secretase subunit APH- 1B, Aph1b ELISA KIT allows for the in vitro quantitative determination of Mouse Aph1b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Gamma- secretase subunit APH- 1B, Aph1b ELISA KIT has been pre-coated with an Mouse Gamma-secretase subunit APH-1B, Aph1b antibody specific to Mouse Aph1b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Aph1b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Aph1b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Aph1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
, macropain) activator subunit 2 (PA28 beta) (PSME2), mRNA 697 NM_002733 Homo sapiens protein kinase, AMP-activated, gamma 1 non-catalytic subunit (PRKAG1), mRNA 497 NM_002631 Homo sapiens phosphogluconate dehydrogenase (PGD), mRNA 1009 NM_002574 Homo
with the recommended amount of ATP, 2 μl of gamma-33P-ATP (20 μCi), and 2 μl of purified enzyme (10 units of cAMP-dependent protein kinase (catalytic subunit), 1000 units of casein kinase II, or 100 units of Erk2). Following the 1 hr incubation, the slides were washed
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
39611β-HSD 1B variantI, active548.0Human serum albuminS, 13.4mg/g biomass536InsulinS, synthetic signal or α-MF, 1.5 g/L, active[233], 532Insulin-like growth factor-1 (IGF-1)S, 600 mg/L, α-MF[234Interferon α-1S, α-MF, 35 mg/L, active520Interferon gamma
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