Mouse Switch- associated protein 70, SWAP70 ELISA KIT ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SWAP70,HSPC321;KIAA0640,RCJMB04_1e1,RCJMB04_8b3, SWAP 70,SWAP-70; switch-associated protein 70; SWAP switching B-cell complex 70kDa subunit
Search name
Mouse SWAP70 ELISA KIT ,Mouse HSPC321 ELISA KIT ,Mouse KIAA0640 ELISA KIT ,Mouse RCJMB04_1e1 ELISA KIT ,Mouse RCJMB04_8b3 ELISA KIT ,Mouse SWAP 70 ELISA KIT ,Mouse SWAP-70 ELISA KIT ,Mouse switch-associated protein 70 ELISA KIT ,Mouse SWAP switching B-cell complex 70kDa subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Switch- associated protein 70, SWAP70 ELISA KIT concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SWAP70, During the reaction, SWAP70 in the sample or standard competes with a fixed amount of biotin-labeled SWAP70 for sites on a pre-coated Monoclonal antibody specific to SWAP70. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SWAP70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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