- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-33979m
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Contactin-1, Cntn1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Contactin- 1, CNTN1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Contactin-1,Glycoprotein gp135,Neural cell surface protein F3, CNTN1,GP135; contactin-1; glycoprotein gP135; neural cell surface protein F3; contactin 1
Search name
Mouse Contactin-1 ELISA KIT ,Mouse Glycoprotein gp135 ELISA KIT ,Mouse Neural cell surface protein F3 ELISA KIT ,Mouse CNTN1 ELISA KIT ,Mouse GP135 ELISA KIT ,Mouse contactin-1 ELISA KIT ,Mouse glycoprotein gP135 ELISA KIT ,Mouse neural cell surface protein F3 ELISA KIT ,Mouse contactin 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Contactin-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Contactin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Contactin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Contactin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Contactin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
每孔上样总蛋白量一致，实现样本间公平对比。 三、实战 protocol：肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后，肿瘤组织 IFN-γ 浓度检测” 为例，操作如下： 1.实验准备：试剂（abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等）、仪器（组织处理器、高速冷冻离心机、酶标仪等）、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解：肿瘤组织离体后用 PBS 冲洗吸干，精准称量 10mg 放入研磨管；加入 100μl 现配
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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