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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
CCR4-NOT transcription complex subunit 4, Cnot4
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse CCR4- NOT transcription complex subunit 4, Cnot4 ELISA KIT
Product Name:Mouse CCR4- NOT transcription complex subunit 4, Cnot4 ELISA KIT
Packing:96T
Catalog No.:ELI-33780m
Gene Name:Mouse Cnot4
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cnot4
Alternative Name:Mouse CCR4-NOT transcription complex subunit 4, Cnot4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse CCR4- NOT transcription complex subunit 4, Cnot4 ELISA KIT allows for the in vitro quantitative determination of Mouse Cnot4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse CCR4- NOT transcription complex subunit 4, Cnot4 ELISA KIT has been pre-coated with an Mouse CCR4-NOT transcription complex subunit 4, Cnot4 antibody specific to Mouse Cnot4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cnot4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cnot4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cnot4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
Platinum Taq PCR x DNA Polymerase (Invitrogen; or equivalent commercial high‐fidelity Taq polymerase kit), including: 10× PCR buffer
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(oligomycin sensitivity conferring protein) (ATP5O), mRNA 1155 NM_001689 Homo sapiens ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9) isoform 3 (ATP5G3), mRNA 1152 NM_001675 Homo sapiens activating transcription factor
VE-1 binding sites (comprising the 9 bp putative interacting sequence and three flanking base pairs) were determined by ELISA and normalized to VE-1 subs. The positions of the potential binding sites relative to the transcription start site
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