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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Ki
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Activated Leukocyte Cell Adhesion Molecule, ALCAM ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
CD166 antigen,Activated leukocyte cell adhesion molecule, ALCAM,CD166; MEMD; CD166 antigen; activated leukocyte cell adhesion
molecule,Protein DM-GRASP,;
Search name
Mouse ALCAM ELISA Kit, Mouse Activated Leukocyte Cell Adhesion Molecule ELISA Kit,
Mouse CD166 antigen ELISA Kit, Mouse CD166 ELISA Kit, Mouse MEMD ELISA Kit, Mouse CD166 antigen ELISA Kit, Mouse
Protein DM-GRASP ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Activated Leukocyte Cell Adhesion Molecule,ALCAM
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALCAM antigen. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ALCAM antigen and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain ALCAM antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ALCAM antigen in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
:
:
: 3128136151
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