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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Carbohydrate sulfotransferase 15, Chst15
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Carbohydrate sulfotransferase 15, Chst15 ELISA KIT
Product Name:Mouse Carbohydrate sulfotransferase 15, Chst15 ELISA KIT
Packing:96T
Catalog No.:ELI-33709m
Gene Name:Mouse Chst15
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Chst15
Alternative Name:Mouse Carbohydrate sulfotransferase 15, Chst15
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Carbohydrate sulfotransferase 15, Chst15 ELISA KIT allows for the in vitro quantitative determination of Mouse Chst15 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Carbohydrate sulfotransferase 15, Chst15 ELISA KIT has been pre-coated with an Mouse Carbohydrate sulfotransferase 15, Chst15 antibody specific to Mouse Chst15 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Chst15 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Chst15 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Chst15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
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