- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-33576m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Chymotrypsinogen B, Ctrb1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Chymotrypsinogen B, Ctrb1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
CTRB1,CTRB; chymotrypsinogen B1, Chymotrypsinogen 2
Search name
Mouse CTRB1 ELISA KIT ,Mouse CTRB ELISA KIT ,Mouse chymotrypsinogen B1 ELISA KIT ,Mouse Chymotrypsinogen 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Chymotrypsinogen Bconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chymotrypsinogen B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Chymotrypsinogen B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chymotrypsinogen B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Chymotrypsinogen B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
30% stock Peroxide 1:100 in di-H2O. 6.OPD Stock, 4.0% 4 g OPD in 100 ml di-H2O. Aliquot and store at -20oC. Protect from light. 7.4.5N H2SO4 12.0 ml Concentrated Sulfuric Acid 88.0 ml di-H2O B. Procedure 1.Wash ELISA plates
Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x
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