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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Voltage-dependent calcium channel subunit alpha-2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Voltage- dependent calcium channel subunit alpha- 2/ delta- 2, CACNA2D2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CACNA2D2,CACNA2D; alpha 2 delta calcium channel subunit; gene 26; voltage-dependent calcium channel subunit alpha-2/delta-2; voltage-gated calcium channel subunit alpha-2/delta-2; calcium channel, voltage-dependent, alpha 2/delta subunit 2
Search name
Mouse CACNA2D2 ELISA KIT ,Mouse CACNA2D ELISA KIT ,Mouse alpha 2 delta calcium channel subunit ELISA KIT ,Mouse gene 26 ELISA KIT ,Mouse voltage-dependent calcium channel subunit alpha-2/delta-2 ELISA KIT ,Mouse voltage-gated calcium channel subunit alpha-2/delta-2 ELISA KIT ,Mouse calcium channel voltage-dependent alpha 2/delta subunit 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Voltage- dependent calcium channel subunit alpha- 2/delta- 2, CACNA2D2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CACNA2D2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CACNA2D2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CACNA2D2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CACNA2D2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Characterization of Calcium Channel Binding
.T., Naylor, M.J., Maybaum, T.A., Pearce, W.G., Koop, B., Fishman, G.A., Mets, M., Musarella, M.A., and Boycott, K.M. 1998. Loss‐of‐function mutations in a calcium‐channel alpha1‐subunit gene in Xp11.23 cause incomplete X‐linked congenital stationary
Purification and Structure of L-Type Calcium Channels
Calcium channels are an essential part of the cellular signal trans duction system, since they produce changes in cytosolic calcium. Three types of voltage-dependent calcium channels (T, L, and N channels) have been identified
subunit of the human brain large-conductance, voltage-activated, calcium-sensitive potassium channel (BK channel) are loaded with Rb+ , washed, and then incubated under experimental conditions. The cell supernatant is removed, and the remaining cell
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