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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Activation-induced cytidine deaminase, Aicda
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Activation- induced cytidine deaminase, AICDA ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
AICDA,activation induced cytidine deaminase,AID; ARP2; CDA2; HEL-S-284; HIGM2; cytidine aminohydrolase; epididymis secretory protein Li 284; integrated into Burkitt's lymphoma cell line Ramos; single-stranded DNA cytosine deaminase; activation-induced cytidine deaminase
Search name
Mouse AICDA ELISA KIT ,Mouse activation induced cytidine deaminase ELISA KIT ,Mouse AID ELISA KIT ,Mouse ARP2 ELISA KIT ,Mouse CDA2 ELISA KIT ,Mouse HEL-S-284 ELISA KIT ,Mouse HIGM2 ELISA KIT ,Mouse cytidine aminohydrolase ELISA KIT ,Mouse epididymis secretory protein Li 284 ELISA KIT ,Mouse integrated into Burkitt's lymphoma cell line Ramos ELISA KIT ,Mouse single-stranded DNA cytosine deaminase ELISA KIT ,Mouse activation-induced cytidine deaminase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Activation- induced cytidine deaminase, AICDA concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to AICDA, During the reaction, AICDA in the sample or standard competes with a fixed amount of biotin-labeled AICDA for sites on a pre-coated Monoclonal antibody specific to AICDA. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AICDA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Performing Bronchoalveolar Lavage in the Mouse
. Activation of the lung S1P1 receptor reduces allergen‐induced plasma leakage in mouse. Brit. J. Pharmacol. 158:1295‐1301. Delayre‐Orthez, C., Becker, J., De Blay, F., Frossard, N
Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System
of activation-induced cytidine deaminase, an enzyme that deaminates cytosine in the context of single-stranded DNA. The chicken DT40 B-cell line is an important model system for exploring the mechanisms of SHM and GCV, as both processes occur constitutively
cells derived from naive mice with the immunogen of interest. Herein, the determination of exclusive panels of cytokines by the ELISA, ELISpot, and FACS technology will serve as an indicator for the activation of defined arms of the immune
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