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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Ankrd43
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Ankrd43 ELISA KIT
Product Name:Mouse Ankrd43 ELISA KIT
Packing:96T
Catalog No.:ELI-34540m
Gene Name:Mouse Ankrd43
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Ankrd43
Alternative Name:Mouse Ankrd43
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ankrd43 ELISA KIT allows for the in vitro quantitative determination of Mouse Ankrd43 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ankrd43 ELISA KIT has been pre-coated with an Mouse Ankrd43 antibody specific to Mouse Ankrd43 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ankrd43 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ankrd43 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ankrd43 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人脑神经相关生长蛋白-43(GAP-43)ELISA试剂盒 说明书
人脑神经相关生长蛋白-43 ( GAP-43 )ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 GAP-43 单抗包被于酶标板上,标准品和样品中的 GAP-43与单抗结合,加入生物素化的抗人 GAP-43 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450nm 处测 OD 值,GAP-43
产生假阳性反应。一般说来,在 3 天内测定的血清标本可放置于 4℃,超过一周测定的需低温冰存。冻结血清融解后,蛋白质局部浓缩,分布不均,应充分混匀宜轻缓,避免气泡,可上下颠倒混和,不要在混匀器上强烈振荡。混浊或有沉淀的血清标本应先离心或过滤,澄清后再检测。反复冻融会使抗体效价跌落,所以测抗体的血清标本如需保存作多次检测,宜少量分装冰存。保存血清自采集时就应注意无菌操作,也可加入适当防腐剂。 2. 试剂的准备 按试剂盒说明书的要求准备实验中需用的试剂。 ELISA 中用的蒸馏水或去离子
。ELISA作为一种固相免疫测定,抗原抗体的结合反应在固相上进行,要使液相中的抗原或抗体与固相上的特异抗体或抗原完全结合,必须在一定的温度条件下反应一定的时间。温育所需时间与温度成反比,即温度越高,则所需时间相对较短。最为常用的温育温度有37℃和室温,其次是43 ℃和2~8 ℃。 温育这一步是临床ELISA测定中最容易出现问题的步骤。通常目前国内ELISA商品试剂盒的反应温育时间为37 ℃ 30分钟~1小时,进口ELISA试剂盒则通常为37 ℃ 1~2小时才能有较完全的结合,低于1小时,可能会影响测定
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