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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Polycomb protein EED, Eed
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Polycomb protein EED, Eed ELISA KIT
Product Name:Mouse Polycomb protein EED, Eed ELISA KIT
Packing:96T
Catalog No.:ELI-32723m
Gene Name:Mouse Eed
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Eed
Alternative Name:Mouse Polycomb protein EED, Eed
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Polycomb protein EED, Eed ELISA KIT allows for the in vitro quantitative determination of Mouse Eed concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Polycomb protein EED, Eed ELISA KIT has been pre-coated with an Mouse Polycomb protein EED, Eed antibody specific to Mouse Eed .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eed and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eed , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eed in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
) Antibody directed against protein of interest (e.g., Upstate Biotechnology, Santa Cruz Biotech, Abcam) Triton X‐100
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
2,EED和SUZ12的PRC2蛋白复合体,介导组蛋白H3在第27位赖氨酸残基的三甲基化修饰(H3K27me3)和转录抑制。与此相反,高的p42/p30比例则导致p42C/EBPa结合到CTNNA1基因的近端启动子元件,通过促进H3K4三甲基化(H3K4me3)修饰激活CTNNA1转录。进一步研究发现,在PTEN敲除的小鼠骨髓和Pten敲低的斑马鱼胚胎中,野生型C/EBPa和alpha-catenin蛋白水平均显著下调,同时伴有造血干祖细胞的白血病样增生和异常浸润。更重要的是,该研究发现临床上约20%
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