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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Epiplakin, Eppk1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Epiplakin, Eppk1 ELISA KIT
Product Name:Mouse Epiplakin, Eppk1 ELISA KIT
Packing:96T
Catalog No.:ELI-30836m
Gene Name:Mouse Eppk1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Eppk1
Alternative Name:Mouse Epiplakin, Eppk1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Epiplakin, Eppk1 ELISA KIT allows for the in vitro quantitative determination of Mouse Eppk1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Epiplakin, Eppk1 ELISA KIT has been pre-coated with an Mouse Epiplakin, Eppk1 antibody specific to Mouse Eppk1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eppk1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eppk1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eppk1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
每孔上样总蛋白量一致,实现样本间公平对比。 三、实战 protocol:肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后,肿瘤组织 IFN-γ 浓度检测” 为例,操作如下: 1.实验准备:试剂(abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等)、仪器(组织处理器、高速冷冻离心机、酶标仪等)、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解:肿瘤组织离体后用 PBS 冲洗吸干,精准称量 10mg 放入研磨管;加入 100μl 现配
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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