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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
SH2 domain-containing protein 3C, Sh2d3c
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse SH2 domain- containing protein 3C, Sh2d3c ELISA KIT
Product Name:Mouse SH2 domain- containing protein 3C, Sh2d3c ELISA KIT
Packing:96T
Catalog No.:ELI-30372m
Gene Name:Mouse Sh2d3c
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Sh2d3c
Alternative Name:Mouse SH2 domain-containing protein 3C, Sh2d3c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse SH2 domain- containing protein 3C, Sh2d3c ELISA KIT allows for the in vitro quantitative determination of Mouse Sh2d3c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse SH2 domain- containing protein 3C, Sh2d3c ELISA KIT has been pre-coated with an Mouse SH2 domain-containing protein 3C, Sh2d3c antibody specific to Mouse Sh2d3c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sh2d3c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sh2d3c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sh2d3c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验BIAcore Analysis to Test Phosphopeptide-SH2 Domain Interactions
long and the three-dimensional structure of several SH2-containing molecules has been resolved. As it is precisely controlled by a subtle balance between protein tyrosine kinases (which phosphorylate) and protein tyrosine phosphatases
Analysis of SH2 Ligands and Identification of Sites of Interaction
complexes. Src homology 2 (SH2) domains are protein modules of approx 100 amino acids that recognize and bind to specific phosphotyrosine-containing polypeptide sequences (1 ). SH2 domains are recruited to tyrosine-phosphorylated proteins in their normal
protein SRC homology 2(SH2) protein of Beta-cells (SHB) for the development of both pancreatic β-cells and blood vessels. SHB is an SH2 domain-containing adapter protein involved in the generation of signaling complexes in response to activation
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