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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Retinoic acid receptor RXR-beta, Rxrb
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Retinoic acid receptor RXR- beta, Rxrb ELISA KIT
Product Name:Mouse Retinoic acid receptor RXR- beta, Rxrb ELISA KIT
Packing:96T
Catalog No.:ELI-30216m
Gene Name:Mouse Rxrb
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Rxrb
Alternative Name:Mouse Retinoic acid receptor RXR-beta, Rxrb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Retinoic acid receptor RXR- beta, Rxrb ELISA KIT allows for the in vitro quantitative determination of Mouse Rxrb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Retinoic acid receptor RXR- beta, Rxrb ELISA KIT has been pre-coated with an Mouse Retinoic acid receptor RXR-beta, Rxrb antibody specific to Mouse Rxrb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rxrb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rxrb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rxrb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Preparation of Polyclonal Antibodies to Retinoid Receptors
of a retinoid “X” receptor α (RXRα) antibody on Western blots contributed to the identification of the RXR ligand by detection of the receptor in fractions of whole-cell lysates that were able to bind the 9-cis retinoic acid ligand (2 ) Antibodies
Gene Targeting of Retinoid Receptors
Gene targeting in embryonic stem (ES) cells is a powerful technique for the modification of the mouse genome (1 –3 ). Regarding the retinoid receptors, a number of laboratories have reported the phenotype of mice in which a given retinoic acid
In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors
acid receptor (RAR), retinoid X receptor (RXR), cellular retinol-binding protein (CRBP), and cellular retinotc acid binding protein (CRABP) gene transcripts. ISH studies have thus revealed that most of the retinoid receptor genes have complex
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