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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Spectrin beta chain, brain 1, Sptbn1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Spectrin beta chain, brain 1, Sptbn1 ELISA KIT
Product Name:Mouse Spectrin beta chain, brain 1, Sptbn1 ELISA KIT
Packing:96T
Catalog No.:ELI-30047m
Gene Name:Mouse Sptbn1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Sptbn1
Alternative Name:Mouse Spectrin beta chain, brain 1, Sptbn1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Spectrin beta chain, brain 1, Sptbn1 ELISA KIT allows for the in vitro quantitative determination of Mouse Sptbn1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Spectrin beta chain, brain 1, Sptbn1 ELISA KIT has been pre-coated with an Mouse Spectrin beta chain, brain 1, Sptbn1 antibody specific to Mouse Sptbn1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sptbn1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sptbn1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sptbn1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
the results. ・ Treptavidin/Beta-Galactosidase Immunohistochemical Labeling Procedure (KPL) Immunohistochemical Staining of Floating Thick Sections (10-100 microns) (KPL) General Protocol for staining thick sections Immunohistochemical
Antigen Design Sera Purification Tech Sheet
that are not contiguous but are brought together by folding of the peptide chain or by the juxtaposition of two separate polypeptide chains. Anti-peptide antibodies may or may not recognize this class of epitope depending on whether the peptide used for antisera
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