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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Threonyl-tRNA synthetase, mitochondrial, Tars2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Threonyl- tRNA synthetase, mitochondrial, Tars2 ELISA KIT
Product Name:Mouse Threonyl- tRNA synthetase, mitochondrial, Tars2 ELISA KIT
Packing:96T
Catalog No.:ELI-29999m
Gene Name:Mouse Tars2
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Tars2
Alternative Name:Mouse Threonyl-tRNA synthetase, mitochondrial, Tars2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Threonyl- tRNA synthetase, mitochondrial, Tars2 ELISA KIT allows for the in vitro quantitative determination of Mouse Tars2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Threonyl- tRNA synthetase, mitochondrial, Tars2 ELISA KIT has been pre-coated with an Mouse Threonyl-tRNA synthetase, mitochondrial, Tars2 antibody specific to Mouse Tars2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tars2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tars2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tars2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验for at least 2 hours at RT on a rotating wheel (or overnight at 4°C); Incubate tissues for at least 1 hour at RT in 1ml of PBS-Tr (PBS 1X; 0.3% Triton X-100) (see comment 2); Transfer tissues into a pre-Hybridization Mixture (pHM) by passing
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), mRNA 502 NM_001537 Homo sapiens heat shock factor binding protein 1 (HSBP1), mRNA 874 NM_003680 Homo sapiens tyrosyl-tRNA synthetase (YARS), mRNA 535 NM_003345 Homo sapiens ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) (UBE2I), mRNA
for mitochondrial proteins 117. MITOP http://www.mips.biochem.mpg.de/proj/medgen/mitop/ Mitochondrial proteins, genes and diseases 118. MOsDB http://mips.gsf.de/proj/rice Oryza sativa genome 119. Mouse Genome Database (MGD) http://www.informatics.jax.org
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